imagej - Image Stack Color Analysis -


i have few images of same rock each of them revealing presence of element in different color. 1 of them show bright green when silicon present, may show dark red calcium present, etc.

is there way overlay these , able tell concentration of minerals @ given point on rock?

is there way overlay these

if have 7 or fewer images, can open images , make them single image stack using image > stacks > images stack. or open them using file > import > image sequence if numbered. or if rgb, can blend them using image calculator (process > image calculator). if end single stack, can use imagej's composite image feature (image > color > make composite) overlay colors.

and able tell concentration of minerals @ given point on rock?

that part harder. may face of same challenges of colocalization analysis. qualitative analysis subjective, , human eyes fooled. see why scatter plots instead of colour merge images on fiji wiki more information.


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